The Mitogen-Activated Protein Kinase CgMK1 Governs Appressorium Formation, Melanin Synthesis, and Plant Infection of Colletotrichum gloeosporioides.
Identifieur interne : 001167 ( Main/Exploration ); précédent : 001166; suivant : 001168The Mitogen-Activated Protein Kinase CgMK1 Governs Appressorium Formation, Melanin Synthesis, and Plant Infection of Colletotrichum gloeosporioides.
Auteurs : Puhuizhong He [République populaire de Chine] ; Yonglin Wang [République populaire de Chine] ; Xiaolian Wang [République populaire de Chine] ; Xiaolin Zhang [République populaire de Chine] ; Chengming Tian [République populaire de Chine]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2017.
Abstract
The fungus Colletotrichum gloeosporiodes infects plant hosts with a specialized cell called an appressorium, which is melanized and required for plant cell wall penetration. Here, we show that the mitogen-activated protein kinase CgMK1 governs appressorium formation and virulence in the poplar anthracnose fungus C. gloeosporioides. Deletion of CgMK1 impairs aerial hyphal growth and biomass accumulation, and CgMK1 is responsible for the expression of melanin biosynthesis-associated genes. CgMK1 deletion mutants are unable to form appressorium and lose the capacity to colonize either wounded or unwounded poplar leaves, leading to loss of virulence. We demonstrate that the exogenous application of cAMP fails to restore defective appressorium formation in the CgMK1 deletion mutants, suggesting that CgMK1 may function downstream or independent of a cAMP-dependent signal for appressorium formation. Moreover, CgMK1 mutants were sensitive to high osmosis, indicating that CgMK1 plays an important role in stress response. We conclude that CgMK1 plays a vital role in regulating appressorium formation, melanin biosynthesis, and virulence in C. gloeosporiodes.
DOI: 10.3389/fmicb.2017.02216
PubMed: 29176970
PubMed Central: PMC5686099
Affiliations:
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Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Yonglin" sort="Wang, Yonglin" uniqKey="Wang Y" first="Yonglin" last="Wang">Yonglin Wang</name><affiliation wicri:level="3"><nlm:affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Xiaolian" sort="Wang, Xiaolian" uniqKey="Wang X" first="Xiaolian" last="Wang">Xiaolian Wang</name><affiliation wicri:level="3"><nlm:affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Xiaolin" sort="Zhang, Xiaolin" uniqKey="Zhang X" first="Xiaolin" last="Zhang">Xiaolin Zhang</name><affiliation wicri:level="3"><nlm:affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Tian, Chengming" sort="Tian, Chengming" uniqKey="Tian C" first="Chengming" last="Tian">Chengming Tian</name><affiliation wicri:level="3"><nlm:affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The fungus <i>Colletotrichum gloeosporiodes</i> infects plant hosts with a specialized cell called an appressorium, which is melanized and required for plant cell wall penetration. Here, we show that the mitogen-activated protein kinase <i>CgMK1</i> governs appressorium formation and virulence in the poplar anthracnose fungus <i>C. gloeosporioides</i>. Deletion of <i>CgMK1</i> impairs aerial hyphal growth and biomass accumulation, and <i>CgMK1</i> is responsible for the expression of melanin biosynthesis-associated genes. <i>CgMK1</i> deletion mutants are unable to form appressorium and lose the capacity to colonize either wounded or unwounded poplar leaves, leading to loss of virulence. We demonstrate that the exogenous application of cAMP fails to restore defective appressorium formation in the <i>CgMK1</i> deletion mutants, suggesting that <i>CgMK1</i> may function downstream or independent of a cAMP-dependent signal for appressorium formation. Moreover, <i>CgMK1</i> mutants were sensitive to high osmosis, indicating that <i>CgMK1</i> plays an important role in stress response. We conclude that <i>CgMK1</i> plays a vital role in regulating appressorium formation, melanin biosynthesis, and virulence in <i>C. gloeosporiodes</i>.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29176970</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>The Mitogen-Activated Protein Kinase <i>CgMK1</i> Governs Appressorium Formation, Melanin Synthesis, and Plant Infection of <i>Colletotrichum gloeosporioides</i>.</ArticleTitle><Pagination><MedlinePgn>2216</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2017.02216</ELocationID><Abstract><AbstractText>The fungus <i>Colletotrichum gloeosporiodes</i> infects plant hosts with a specialized cell called an appressorium, which is melanized and required for plant cell wall penetration. Here, we show that the mitogen-activated protein kinase <i>CgMK1</i> governs appressorium formation and virulence in the poplar anthracnose fungus <i>C. gloeosporioides</i>. Deletion of <i>CgMK1</i> impairs aerial hyphal growth and biomass accumulation, and <i>CgMK1</i> is responsible for the expression of melanin biosynthesis-associated genes. <i>CgMK1</i> deletion mutants are unable to form appressorium and lose the capacity to colonize either wounded or unwounded poplar leaves, leading to loss of virulence. We demonstrate that the exogenous application of cAMP fails to restore defective appressorium formation in the <i>CgMK1</i> deletion mutants, suggesting that <i>CgMK1</i> may function downstream or independent of a cAMP-dependent signal for appressorium formation. Moreover, <i>CgMK1</i> mutants were sensitive to high osmosis, indicating that <i>CgMK1</i> plays an important role in stress response. We conclude that <i>CgMK1</i> plays a vital role in regulating appressorium formation, melanin biosynthesis, and virulence in <i>C. gloeosporiodes</i>.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>Puhuizhong</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yonglin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiaolian</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xiaolin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Chengming</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Forestry, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Colletotrichum gloeosporiodes</Keyword><Keyword MajorTopicYN="N">MAPK</Keyword><Keyword MajorTopicYN="N">appressorium formation</Keyword><Keyword MajorTopicYN="N">penetration</Keyword><Keyword MajorTopicYN="N">virulence</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>08</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>10</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29176970</ArticleId><ArticleId 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